Facile fabrication of microperforated membranes with re-useable SU-8 molds for organs-on-chips
نویسندگان
چکیده
Microperforated membranes are essential components of various organ-on-a-chip (OOC) barrier models developed to study transport molecular compounds and cells across cell layers in e.g. the intestine blood-brain barrier. These OOC have two functions: 1) support growth on one or both sides, 2) act as a filter-like separate adjacent compartments. Thin, microperforated poly(dimethylsiloxane) (PDMS) can be fabricated by micromolding from silicon molds comprising arrays micropillars for formation micropores. However, these made deep reactive ion etching (DRIE) expensive fabricate. We describe thin PDMS with easier-to-make, SU-8 epoxy photoresist molds. With multilayer, SU-8, pillar microarray mold, massively parallel micropores formed layer PDMS, resulting flexible membrane that easily incorporated sealed between other making up device. The we here 30-μm thickness, 12-μm-diameter circular pores arranged at 100-μm pitch square array. show application gut-on-a-chip devices, expect reported fabrication strategy will also suitable dimensions.
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ژورنال
عنوان ژورنال: Organs-on-a-chip
سال: 2023
ISSN: ['2666-1020']
DOI: https://doi.org/10.1016/j.ooc.2023.100026